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The Nile on eBay The Polymerase Chain Reaction by J.D. Watson This text on the polymerase chain reaction (PCR) examines the most up-to-date methodological protocols. Included are new techniques and enhanced methods that show the novice and experienced PCR user how to optimize results. Future enhancements and new potential uses of PCR are discussed. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description Edited by the inventor of PCR and two prominent experts in PCR techniques, this first comprehensive handbook on the Polymerase Chain Reaction has the most up to date methodological protocols from the world's leading laboratories. Included are exciting new techniques and enhanced methods, previously unavailable in book form, that show the novice and experienced PCR user exactly how they can optimize their results. The applications chapters are quite unique, with the foremost researchers providing not only protocols, but explaining why PCR has revolutionized their particular field. Future enhancements of PCR as well as new potential uses are discussed. Readers will learn how PCR has changed the face of diagnostic testing, cancer research, genetics, forensics, plant biology, DNA sequencing, gene therapy, and much more! Nearly forty chapters have been extensively reviewed and checked for accuracy and breadth of subject matter. Table of Contents Methodology; manipulation of DNA, K. Hayashi; cloning PCR products, M. Frohman; multiplex PCRs, J.S. Chamberlain & J.R. Chamberlain; preparation of nucleic acids, D. Shibata; amplification of viral DNA and viral host cell mRNAs in situ, J. Embretson et al; quantitation - an overview, F. Ferre et al; quantification of DNAs by the polymerase chain using an internal control, D.M. Coen; RT-PCR and mRNA quantitation, J. Chelly and A. Kahn; analysis of T-cell repertoires by PCR, D. Spinella and J.M. Robertson; ultra-sensitive non-radioactive detection of PCR products - an overview, R.H. Tullis; fluorescent detection methods for PCR analysis, M.J. Heller; enzyme-labeled oligonucleotides, E. Tu & E. Jablonski; application of the hybridization protection assay to PCR, N.C. Nelson & S.H. McDonough; instrumentation - an overview, C. Oste; rapid cycle DNA amplification, C.T. Wittwer et al; automating PCR, H.R. Gardner; sequencing - an overview, R.A. Gibbs; and applications chapters on PCR and cancer, diagnostics, gene therapy, plant biology, forensics, and much more. Details ISBN 0817636072 Pages 600 Year 1994 ISBN-10 0817636072 ISBN-13 9780817636074 Format Hardcover Edited by R. Gibbs DEWEY 574.873282 Publisher Birkhauser Boston Inc Imprint Birkhauser Boston Inc Place of Publication Secaucus Country of Publication United States Short Title POLYMERASE CHAIN REACTION Language English Media Book Author J.D. Watson Edition 94199th Publication Date 1994-12-31 Subtitle A Textbook DOI 10.1007/b54114 Audience College/Higher Education We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:159632990;