Description

500 units of Taq DNA polymerase for PCR amplification. Full refund if not satisfied. Shippind Fedex 2 -nd day $21(with coolant) or USPS Priority mail $7 (no coolant (no visible lost of activity at 37oC for one week)). We accept Credit card payments. Taq DNA polymerase is a thermostable DNA polymerase purified from the Thermus aquaticus strain in accordance with the procedures developed by Kaledin (1,2,3) for the isolation of thermostable enzymes processing DNA polymerase activity from thermophilic bacteria. Amplification of DNA fragments (100 bp to 10kb) can be achieved with this enzyme. The enzyme has both 5'-3' polymerase- and 5'-3' exonuclease activities. Taq Polymerase can add a single template-directed deoxyadenosin (A) residue to the 3’ end of duplex PCR products. CONCENTRATION: 5 units/µl UNIT DEFINITION: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic acid, sodium salt) pH 9.3 (at 25°C); 50 mM KCl; 2 mM MgCl2; 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate. STORAGE BUFFER: 10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol(v/v) STORAGE TEMPERATURE: Store Taq DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer. 10X REACTION BUFER (not complete) : 160 mM (NH 4 ) 2 SO 4 , 670 mM Tris-HCl pH 8.8 (at 25°C) , 0.1% Tween 20 The descriptions and photos represent best efforts to convey information and our opinions about a product. The items in this listing are being sold irrespective of condition, wear, or damage, and with all faults, defects, and with no representations, guarantees or warranties of any kind about the item's functionality beyond what is in the description. Sibgene LLC EXPRESSLY DISCLAIMS ANY AND ALL WARRANTIES OR MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. We expect some items to have limited functionality and/or require repairs. The listed price takes this into consideration and we ask buyers to take these purchase terms into account. Items without best offer means that we are not accepting offers unless there are multiple items or combined purchases. Purchaser agrees that seller is not liable for any injuries or damages of any kind associated in any way with this equipment. Purchaser agrees to indemnify and hold harmless seller and seller's officers, directors, employees, agents, and shareholders against any and all claims, liabilities, obligations, demands, cost and expenses of every kind and nature, including but not limited to attorney's fees and litigation expenses, from or related to the equipment and any use thereof. Please contact the seller if you would like additional information about an item.