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The Nile on eBay Quantitation of mRNA by Polymerase Chain Reaction by Thomas Köhler, Dirk Laßner, Anne-Katrin Rost, Barbara Thamm, Barbara Pustowoit, Harald Remke In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research. Notes In this laboratoy "cook-book", the authors provide a concise guide to PCR-based quantification of nucleic acids using exclusivley nonradioactive detection. The procedures described represent state-of-the-art know-how and are presented in a very detailed and practice-oriented manner. Thus, this laboratory guide enables professionals and beginners to adopt easily quantitative PCR protocols into their own research. Back Cover In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research. Table of Contents I Theoretical and Methodical Prerequisites for Using PCR to Quantitate Nucleic Acids.- 1.1 General Aspects and Chances of Nucleic Acid Quantitation by PCR.- 1.2 Design of Suitable Primers and Competitor Fragments for Quantitative PCR.- 1.3 Cloning of Short DNA Fragments and In Vitro Transcription to Generate RNA Standards.- 1.4 Direct Non-lsotopic Sequencing of PCR Products or Standards.- 2Conventional Techniques for mRNA Analysis.- 2.1 Isolation of mRNA.- 2.2 Synthesis of cDNA.- 2.3 Qualitative RT-PCR: Amplification of Synthesized mdr-1 cDNA.- 2.4 Single-Tube RT-PCR.- 2.5 Nonradioactive Determination of PCR Products by Using a DIG-Labeled DNA Probe (Dot Blot).- 2.6 Nonradioactive Northern Blot Hybridization with DIG-Labeled DNA Probes.- 3 Semiquantitative and Quantitative Protocols for Measurement of Nucleic Acids by PCR.- 3.1 Quantitation of mRNA by the ELOSA Technique Using External Standards.- 3.2 Semiquantitative Detection of Viral DNA, e.g. for CMV, by Using the DNA Enzyme Immunoassay (DEIA).- 3.3 HPLC-Analysis of Nucleic Acids.- 3.4 Quantitation of Absolute Numbers of mRNA Copies in a cDNA Sample by Competitive PCR.- Acknowledgment. Review "...this technical book will be very useful for all customers of PCR. And they are numerous!... Summarizing: an excellent booklet, which we recommend highly and absolutely necessary to all practicians of PCR products and their applications." Cellular and Molecular Biology Promotional Springer Book Archives Long Description In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research. Review Quote "...this technical book will be very useful for all customers of PCR. And they are numerous!... Summarizing: an excellent booklet, which we recommend highly and absolutely necessary to all practicians of PCR products and their applications." Cellular and Molecular Biology Description for Sales People In this laboratoy "cook-book", the authors provide a concise guide to PCR-based quantification of nucleic acids using exclusivley nonradioactive detection. The procedures described represent state-of-the-art know-how and are presented in a very detailed and practice-oriented manner. Thus, this laboratory guide enables professionals and beginners to adopt easily quantitative PCR protocols into their own research. Details ISBN 3642797148 Short Title QUANTITATION OF MRNA BY POLYME Series Springer Lab Manuals Language English ISBN-10 3642797148 ISBN-13 9783642797149 Media Book Format Paperback Year 2011 Publication Date 2011-12-29 Imprint Springer-Verlag Berlin and Heidelberg GmbH & Co. K Place of Publication Berlin Country of Publication Germany Pages 166 Subtitle Nonradioactive PCR Methods DOI 10.1007/978-3-642-79712-5 Author Harald Remke Publisher Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Alternative 9783540591924 DEWEY 574.873283 Illustrations 17 Illustrations, black and white; XX, 166 p. 17 illus. Audience Professional & Vocational Edition Description Softcover reprint of the original 1st ed. 1995 We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96327397;