Description
The Nile on eBay Molecular Biology Techniques by Beatriz Dantas Guimarães The polymerase chain reaction (PCR) has as its main application the possibility of amplifying a segment of DNA, producing millions of copies of the selected segment. Gene cloning aims to introduce a selected gene into bacteria that are identical to each other, and this technique requires the presence of a cloning vector capable of amplifying a fragment of DNA cloned into them. The E gene of the dengue virus (DENV) is the most widely used for molecular studies due to the importance of the E protein. The aim of this study is to establish techniques for amplifying the E gene of DENV-2 and DENV-4. The DNA molecule was obtained from FIOCRUZ in Recife; the primers were selected in such a way as to encompass the E gene. PCR amplification was satisfactorily established for the E gene. Once determined, these techniques are essential in the stages of obtaining recombinant DNA in the process of cloning and expressing the dengue E protein. FORMAT Paperback CONDITION Brand New Details ISBN 6208515491 Author Beatriz Dantas Guimarães Pages 52 Publisher Our Knowledge Publishing Year 2024 ISBN-13 9786208515492 Format Paperback Publication Date 2024-12-30 Imprint Our Knowledge Publishing Subtitle Polymerase Chain Reaction (PCR) Audience General We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:170930646;
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