Description
The polymerase chain reaction (PCR) has as its main application the possibility of amplifying a segment of DNA, producing millions of copies of the selected segment. Gene cloning aims to introduce a selected gene into bacteria that are identical to each other, and this technique requires the presence of a cloning vector capable of amplifying a fragment of DNA cloned into them. The E gene of the dengue virus (DENV) is the most widely used for molecular studies due to the importance of the E protein. The aim of this study is to establish techniques for amplifying the E gene of DENV-2 and DENV-4. The DNA molecule was obtained from FIOCRUZ in Recife; the primers were selected in such a way as to encompass the E gene. PCR amplification was satisfactorily established for the E gene. Once determined, these techniques are essential in the stages of obtaining recombinant DNA in the process of cloning and expressing the dengue E protein.
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Communication Prior to Buying was Responsive and Quick. However, having been Reassured that My Order would be Delivered by 'Local Postal Service', it Arrived by Private Courier. The Delivery itself took 25 Days to Arrive. Although it was Delivered before the Estimated Delivery Date, Locally Based Orders Shouldn't have to Take So Long to be Delivered. The New Item Arrived in Excellent Condition as Described. A Satisfying Buy at a Great Value Price but CS Advice Should've been More Reliable.
Dev1996c743a
This Seller is the Very Best. From First Contact to Delivery no one can surpass how Fantastic they are. Expertly Packed item, Fast Delivery and arrived in Perfect Condition. The price charged was competitive but not greedy and the postage the cost you would expect. They answered any enquiries quickly and full of the right information. I am so very, very pleased with this purchase and the Brrrrrilliant Seller. Thank you so very, very much. Keep doing what you are doing. Fantastic!
Rob Trautvetter
Many thanks, the item arrived very well packaged and in perfect condition. It also arrived ahead of the initial scheduled date. The quality is superb and I think this represents great value for money. Excelent communication from an honest seller. I would definitely recommend this seller to anyone. Once again, many thanks.