Description

High pure Taq DNA polymerase PCR with 500U for Polymer Chain Reaction 500 units of Taq DNA polymerase for PCR amplification. We guarantee that our Taq polymerase is working. We will provide a full refund if you do not satisfy it. Our Taq DNA polymerase has a high purity quality and high activity. We purified it by two systems, affinity and ion exchange. We develop a new purification method. Amplification of DNA fragments (100 bp to 10kb) can be achieved with this enzyme. The enzyme has both 5'-3' polymerase- and 5'-3' exonuclease activities. CONCENTRATION: 5 units/µl UNIT DEFINITION: One unit is defined as the amount of enzyme that incorporates 10 nM of dNTPs into acid in soluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-pro-panesulfonic acid, sodium salt) pH 9.3 (at 25°C); 50 mM KCl; 2 mM MgCl2; 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate. STORAGE BUFFER : 50 mM-Tris-HCl buffer pH 8.0, 100 mM NaCl, 0.1 mM EDTA, 3 mM DTT, 50% glycerol(v/v) STORAGE TEMPERATURE : Store Taq DNA polymerase at -80 ℃ when you do not want to use it, If you often use it keep it at -20 ℃ . 10X REACTION BUFFER (not complete) is the most efficient buffer without a detergent component. Taq polymerase is the most important factor in the PCR reaction but not the only factor. You should check the template, primers, and dNTP. If the template, primers, and dNTP do not have the problems, we’d like to fully refund you. Please contact us if you have questions about the taq polymerase. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5 ´→ 3 ´ polymerase activity (1,2,3) and a 5 ´ flap endonuclease activity (4,5). It is supplied with 10X Taq polymerase Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations. Highlights Isolated from a recombinant source Supplied with 10X Reaction Buffer Robust and reliable reactions Tolerates a wide range of templates Exceptional value in terms of cost per unit Product Source An E. coli strain that carries the Taq DNA Polymerase. This product is related to the following categories: Taq DNA Polymerase Products This product can be used in the following applications: Colony PCR, A-tailing, Polymerases for DNA Manipulation, Multiplex PCR, Specialty PCR, Routine PCR, PCR